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产品目录

小鼠抗PRMT5抗体图片
产品货号:
YT753
中文名称:
小鼠抗PRMT5抗体
英文名称:
PRMT5 Mouse Monoclonal Antibody
产品规格:
50μl
发货周期:
1~3天
产品价格:
询价
本品为来源于小鼠的抗PRMT5的单克隆抗体。
宿主:小鼠
克隆性:单克隆抗体
用途:WB,IHC
交叉反应性:人,小鼠,大鼠
分子量:72KDa
推荐稀释比例:WB(1:1000),IHC(1:100-200)

About this Antibody
Name PRMT5 Mouse Monoclonal Antibody
Category Monoclonal antibody(mAb); Primary antibody
Isotype IgG2a
Purification ProA affinity purified
About the Immunogen
Immunogen This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of PRMT5.
Gene ID 10419
SwissProt O14744
Synonyms HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs; PRMT5; Skb1Hs Methyltransferase
Category Chromatin Regulation / Nuclear Function
Background The formation of the spliceosome includes the assembly of Sm proteins inan ordered manner onto snRNAs. This process is mediated by the survivalof a motor neuron (SMN) protein, and is enhanced by modification of specificArginine residues in the Sm proteins to symmetrical dimethylarginines(sDMAs). sDMA modification of Sm proteins is catalyzed by the methylosome,a complex comprised of the type II methyltransferase PRMT5 (alsodesignated JAK-binding protein 1, JBP1), pICln and two novel factors. PRMT5binds the Sm proteins via their Arginine- and Glycine-rich (RG) domains, whilepICln binds the Sm domains. PRMT5 is a distinct member of the protein-Arginine methyltransferase (PRMT) family, and predominantly localizes tothe cytoplasm in a wide variety of tissues. PRMT5 also associates specificallywith the transcription start site region of the cyclin E1 promoter and, therefore,is involved in the control of transcription and proliferation.The geneencoding human PRMT5 maps to chromosome 14q11.2.


注意事项:
1. 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
2. 回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
3. 提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。

储存条件:-20℃,有效期一年。
相关搜索:小鼠抗PRMT5抗体PRMT5 Mouse Monoclonal Antibody
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